Journal: Advanced Science

Article Title: Loss of Endothelial Annexin A1 Aggravates Inflammation‐Induched Vascular Aging

doi: 10.1002/advs.202307040

Figure Lengend Snippet: Treatment with Ac2‐26 alleviates the aging process in ANXA1 KO mice. A) Ac2‐26 treatment improved the appearance of ANXA1 KO mice. B) The area of positive SA‐β‐gal staining in the aorta was reduced in ANXA1 KO mice treated with Ac2‐26 ( n = 6). C) The PP in ANXA1 KO mice was reduced by Ac2‐26 treatment ( n = 6). D,E) ACH‐induced endothelium‐dependent relaxation of thoracic aortic rings was rescued by Ac2‐26 treatment in KO mice. Maximum relaxation induced by ACH ( n = 6). F,G) The decreased PWV indicated that the degree of aortic stiffness was decreased with the Ac2‐26 treatment compared to that in the ANXA1 KO group ( n = 8). H–K) Collagen accumulation and elastin breaks were also improved by Ac2‐26 with HE staining and VVG staining. Enlargement of the lumen, intima‐media thickening, collagen accumulation, and breaks in elastin were improved after Ac2‐26 treatment in KO mice. The data are presented as the means ±SEMs (C,G) and means ±SDs (D,E,J,K). Two‐way ANOVA followed by the Bonferroni post hoc test (C,E,G,J,K) and multiple repeated measures ANOVA followed by the Bonferroni post hoc test (D) were used for the analyses. Bars = 100 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, p > 0.05.

Article Snippet: Ac2‐26 (MCE, USA) was used at a concentration of 300 µ m before lentiviral infection of HUVECs and was applied until 3 days after infection.

Techniques: Staining

Journal: Advanced Science

Article Title: Loss of Endothelial Annexin A1 Aggravates Inflammation‐Induched Vascular Aging

doi: 10.1002/advs.202307040

Figure Lengend Snippet: Inflammaging in ANXA1 knockdown HUVECs is reversed by Ac2‐26 treatment. A,B) Representative photographs of SA‐β‐gal staining demonstrating that Ac2‐26 can attenuate senescence in HUVECs ( n = 3). C,D) G0/G1 arrest was reversed by Ac2‐26 treatment, as determined by flow cytometric analysis ( n = 3). E) Cumulative population doubling level (CPDL) of the three groups. F,G) Representative western blot images showing that the expression of P21 was reduced by Ac2‐26 treatment ( n = 3). H–J) Representative western blot images showing that the expression of Rb and Cyclin E1 were reduced by Ac2‐26 treatment ( n = 3). K) mRNA transcript levels of SERPINE1, P21, P16, and SASP as the TGFβ2 level decreased with Ac2‐26 treatment, as quantified by qRT‐PCR ( n = 3). L,M) Representative images of the tube formation assay. The number of tubes formed by HUVECs was restored by Ac2‐26 treatment ( n = 5). The data are presented as the means ±SEMs (B,D,E,G,I,J,K) and means ±SDs (M). One‐way ANOVA followed by the Bonferroni post hoc test (B,D,E,G,I,J,K) was used for the analyses. Bars = 200 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, p > 0.05.

Article Snippet: Ac2‐26 (MCE, USA) was used at a concentration of 300 µ m before lentiviral infection of HUVECs and was applied until 3 days after infection.

Techniques: Knockdown, Staining, Western Blot, Expressing, Quantitative RT-PCR, Tube Formation Assay

Journal: Synthetic and Systems Biotechnology

Article Title: Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach

doi: 10.1016/j.synbio.2025.12.001

Figure Lengend Snippet: Schematic overview of the experimental workflow for generating ANXA1-knockdown CHO cell lines and AC2-26 inhibitor treatment. (A) Generation and validation of ANXA1-knockdown cell lines for rADM antibody production. (B) AC2-26 inhibitor treatment in low-producer CHO cells (ADM-14) and subsequent rADM expression analysis.

Article Snippet: On day 3 of suspension culture, the small molecule inhibitor AC2-26 (Topscience Co., Ltd., China) was added [ ], using DMSO (Solarbio Life Sciences, China) as the solvent control.

Techniques: Knockdown, Biomarker Discovery, Expressing

Journal: Synthetic and Systems Biotechnology

Article Title: Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach

doi: 10.1016/j.synbio.2025.12.001

Figure Lengend Snippet: AC2-26 effect on ADM-14 CHO cells. (A) Cell density/viability under AC2-26 treatment (n = 3). (B) rADM expression by Western blot with quantification (n = 3). (C) ANXA1 mRNA/protein comparison (n = 3). Quantification was performed using ImageJ (for Western blot densitometry) and GraphPad Prism 10 (for statistical analysis). (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001). n: represents independent biological replicates.

Article Snippet: On day 3 of suspension culture, the small molecule inhibitor AC2-26 (Topscience Co., Ltd., China) was added [ ], using DMSO (Solarbio Life Sciences, China) as the solvent control.

Techniques: Expressing, Western Blot, Comparison